Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker
This study investigated the protective effects
of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium
strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri
(Xac 306). The LN bacteria strain was cultured in liquid medium and the
supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF),
respectively, and then the extract was concentrated, filtrated, lyophilized and
fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions
were obtained. All fractions’ activity against Xac 306 by agar well diffusion
assay and minimum inhibitory concentration but in different concentrationswere
tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL-1
concentration. The comet assay demonstrated that the fractions EAF, VLC2 and
VLC3 presented no genotoxic effects at tested concentrations. In plants only
VLC3 showed significant results (p<0.05), reducing the incidence of citrus
canker lesions.
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